| Thuis | Uit | Uitslag | Datum |
| Ajax | FC Twente | 3-0 (2-0) | 07 feb 2010 |
| Feyenoord | AZ | 1-2 (1-1) | 07 feb 2010 |
| RKC Waalwijk | NEC | 0-1 (0-0) | 07 feb 2010 |
| VVV-Venlo | Vitesse | 2-0 (1-0) | 07 feb 2010 |
| Heracles Almelo | FC Groningen | 4-3 (0-2) | 06 feb 2010 |
| PSV | ADO Den Haag | 2-0 (1-0) | 06 feb 2010 |
| Roda JC | Sparta Rotterdam | 2-1 (1-0) | 06 feb 2010 |
| sc Heerenveen | FC Utrecht | 2-0 (1-0) | 06 feb 2010 |
| Willem II | NAC Breda | 1-2 (1-1) | 05 feb 2010 |
| RKC Waalwijk | Feyenoord | 0-1 (0-0) | 04 feb 2010 |
| Ajax | Roda JC | 4-0 (0-0) | 03 feb 2010 |
| FC Groningen | ADO Den Haag | 2-0 (1-0) | 03 feb 2010 |
| FC Twente | Heracles Almelo | 2-0 (1-0) | 03 feb 2010 |
| PSV | FC Utrecht | 2-1 (1-1) | 03 feb 2010 |
| Sparta Rotterdam | Vitesse | 1-1 (0-0) | 03 feb 2010 |
| NAC Breda | AZ | 1-1 (0-0) | 02 feb 2010 |
| VVV-Venlo | sc Heerenveen | 3-1 (3-1) | 02 feb 2010 |
|